CLONING OF CDNAS FOR HUMAN PHOSPHORIBOSYLPYROPHOSPHATE SYNTHETASE-1 AND SYNTHETASE-2 AND X-CHROMOSOME LOCALIZATION OF PRPS1 AND PRPS2 GENES

被引：46
作者：
BECKER, MA
HEIDLER, SA
BELL, GI
SEINO, S
LEBEAU, MM
WESTBROOK, CA
NEUMAN, W
SHAPIRO, LJ
MOHANDAS, TK
ROESSLER, BJ
PALELLA, TD
机构：
[1] UNIV CHICAGO,HOWARD HUGHES MED INST,CHICAGO,IL 60637
[2] UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DEPT PEDIAT,TORRANCE,CA 90509
[3] UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DEPT BIOL CHEM,TORRANCE,CA 90509
[4] UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,HOWARD HUGHES MED INST,TORRANCE,CA 90509
[5] UNIV MICHIGAN,DEPT INTERNAL MED,ANN ARBOR,MI 48109
来源：
GENOMICS | 1990年 / 8卷 / 03期
关键词：
D O I：
10.1016/0888-7543(90)90043-T
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cloned cDNAs representing the entire, homologous (80%) translated sequences of human phosphoribosylpyrophosphate synthetase (PRS) 1 and PRS 2 cDNAs were utilized as probes to localize the corresponding human PRPS1 and PRPS2 genes, previously reported to be X chromosome linked. PRPS1 and PRPS2 loci mapped to the intervals Xq22-q24 and Xp22.2-p22.3, respectively, using a combination of in situ chromosomal hybridization and human × rodent somatic cell panel genomic DNA hybridization analyses. A PRPS1-related gene or pseudogene (PRPS1L2) was also identified using in situ chromosomal hybridization at 9q33-q34. Human HPRT and PRPS1 loci are not closely linked. Despite marked cDNA and deduced amino acid sequence homology, human PRS 1 and PRS 2 isoforms are encoded by genes widely separated on the X chromosome. © 1990.
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页码：555 / 561
页数：7
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