THE AVIAN RETROVIRAL IN PROTEIN IS BOTH NECESSARY AND SUFFICIENT FOR INTEGRATIVE RECOMBINATION INVITRO

被引：343

作者：

KATZ, RA ^{[1
]}

MERKEL, G ^{[1
]}

KULKOSKY, J ^{[1
]}

LEIS, J ^{[1
]}

SKALKA, AM ^{[1
]}

机构：

[1] CASE WESTERN RESERVE UNIV,DEPT BIOCHEM,CLEVELAND,OH 44106

来源：

CELL | 1990年 / 63卷 / 01期

关键词：

D O I：

10.1016/0092-8674(90)90290-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The integration of viral DNA into the host cell chromosome is an essential feature of the retroviral life cycle. The integration reaction requires cis-acting sequences at the end of linear viral DNA and a trans-acting product of the pol gene, the integration protein (IN). Previously, we demonstrated that avian sarcoma-leukosis virus (ASLV) IN is able to carry out the first step in the integration process in vitro: nicking of the ends of linear viral DNA. In this paper, using two independent assays, we demonstrate that IN, alone, is sufficient to carry out the second step: cleavage and joining to the target DNA. These results demonstrate that the retroviral IN protein is an integrase. © 1990.

引用

页码：87 / 95

页数：9

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