SYNGENEIC LYSIS OF RETICULOENDOTHELIOSIS VIRUS-TRANSFORMED CELL-LINES TRANSFECTED WITH MAREKS-DISEASE VIRUS GENES BY VIRUS-SPECIFIC CYTOTOXIC T-CELLS

被引:21
作者
UNI, Z
PRATT, WD
MILLER, MM
OCONNELL, PH
SCHAT, KA
机构
[1] CORNELL UNIV, COLL VET MED, DEPT AVIAN & AQUAT ANIM MED, ITHACA, NY 14853 USA
[2] CITY HOPE NATL MED CTR, BECKMAN RES INT, DUARTE, CA 91010 USA
关键词
D O I
10.1016/0165-2427(94)90169-4
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Cell-mediated immune responses against Marek's disease virus (MDV) antigens were examined using reticuloendotheliosis virus (REV)-transformed cell lines of two haplotypes ((BB19)-B-19 and (BB13)-B-13). These cell lines were stably transfected with cloned fragments of MDV DNA resulting in the expression of the MDV-specific phosphoprotein pp38. Effector cells were obtained from P2a ((BB19)-B-19) and S13 ((BB13)-B-13) chickens at 7 days post inoculation with REV, oncogenic or attenuated serotype 1 MDV (JM-16/O and JM-16/A, respectively), serotype 2 MDV (SB-1), or herpesvirus of turkeys (HVT). Transfection of MDV genes did not influence the expression of Class I major histocompatibility complex antigens. The optimal effector to target cell ratio was determined to be 100:1. REV-sensitized effector cells lysed REV cell lines and REV cell lines transfected with MDV DNA in a syngeneic fashion. Effector cells from chickens inoculated with JM-16/O, JM16/A, SB-1 or HVT lysed only the syngeneic, transfected cell lines, but not the parent REV cell lines. The percentage specific release caused by the MDV-sensitized effector cells was low, but statistically significant.
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页码:57 / 69
页数:13
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