MAJOR STRUCTURAL FEATURES OF THE CELL-WALL ARABINOGALACTANS OF MYCOBACTERIUM, RHODOCOCCUS, AND NOCARDIA SPP

被引:86
作者
DAFFE, M
MCNEIL, M
BRENNAN, PJ
机构
[1] COLORADO STATE UNIV,DEPT MICROBIOL,FT COLLINS,CO 80523
[2] CNRS,LPTF,DEPT GLYCOCONJUGUES & BIOMEMBRANES,F-31055 TOULOUSE,FRANCE
关键词
D O I
10.1016/0008-6215(93)84102-C
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cell wall arabinogalactans of strains of Mycobacterium, Rhodococcus, and Nocardia were per-O-methylated, partially hydrolyzed with acid, and the resulting oligosaccharides were reduced and per-O-ethylated to yield per-O-alkylated oligoglycosyl alditol fragments. Analyses of these fragments by gas chromatography-mass spectrometry and of the intact solubilized polysaccharides by H-1 and C-13 NMR revealed the major structural features of the different arabinogalactans from representatives of the different genera. All of the mycobacterial products contained a homogalactan segment of alternating 5-linked alpha-galactofuranosyl (Galf) and 6-linked beta-Galf residues. The arabinan segment consisted of three major domains, linear 5-linked alpha-arabinofuranosyl (Araf) residues and branched (3 --> 5)-linked Araf units substituted with either 5-linked Araf or the disaccharide beta-Araf-(1 --> 2)-alpha-Araf at both branched positions. The recognition of these features in in vivo grown Mycobacterium leprae is an important development. The arabinan from strains of Nocardia contains a nonreducing-end motif composed of the linear trisaccharide, beta-Araf-(1 --> 2)-alpha-Araf-(1 --> 5)-Araf, attached to linear 5-linked alpha-Araf units. The galactan segment of the arabinogalactan of Nocardia sp. is composed of linear 5-linked beta-Galf units substituted in part at O-6 with terminal beta-glucosyl units. The two representative strains of Rhodococcus also differed in the composition of the galactan moiety; in addition to the 5-linked Galf, 2- and 3-linked beta-Galf units are present. The reducing end of the galactans, and therefore, apparently, of the entire arabinogalactans from all species from all genera, are apparently composed of the unit, rhamnosyl-(1 --> 3)-N-acetyl-glucosamine, which, in turn, is apparently attached to peptidoglycan via phosphodiester linkage.
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页码:383 / 398
页数:16
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