ALKALI-INDUCED OPTICAL-ROTATION CHANGES IN HEPARINS AND HEPARAN SULFATES, AND THEIR RELATION TO IDURONIC ACID-CONTAINING SEQUENCES

被引：25

作者：

PIANI, S ^{[1
]}

CASU, B ^{[1
]}

MARCHI, EG ^{[1
]}

TORRI, G ^{[1
]}

UNGARELLI, F ^{[1
]}

机构：

[1] IST SCI CHIM & BIOCHIM G RONZONI,I-20133 MILAN,ITALY

来源：

JOURNAL OF CARBOHYDRATE CHEMISTRY | 1993年 / 12卷 / 4-5期

关键词：

D O I：

10.1080/07328309308019404

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Under specific basic conditions, the glucosaminoglycans heparin and heparan sulfate, containing alpha-L-iduronic acid 2-O-sulfate, undergo selective epoxidation between C-2 and C-3 of this residue, with formation of a residue of 2,3-anhydro-alpha-L-guluronic acid. The epoxidation reaction was studied by means of C-13 NMR and optical rotation measurements. The optical rotation values correlate well with the composition of the reaction products as determined by C-13 NMR, thus indicating that the heparin- or heparan-like character of both natural and semisynthetic polysaccharides can be easily determined also through optical rotation measurements.

引用

页码：507 / 521

页数：15

共 16 条

[1] STRUCTURE AND BIOLOGICAL-ACTIVITY OF HEPARIN [J].