SEQUENCE OF THE GENE ENCODING AN ALKALINE SERINE-PROTEASE OF THERMOPHILIC BACILLUS-SMITHII

被引：4

作者：

MILANO, A

MANACHINI, PL

PARINI, C

RICCARDI, G

机构：

[1] UNIV PAVIA,DIPARTIMENTO GENET & MICROBIOL,I-27100 PAVIA,ITALY

[2] UNIV MILAN,DIPARTIMENTO SCI & TECHNOL ALIMENTARI & MICROBIOL,I-20133 MILAN,ITALY

来源：

GENE | 1994年 / 145卷 / 01期

关键词：

RECOMBINANT DNA; DNA SEQUENCING; PCR; INVERSE PCR;

D O I：

10.1016/0378-1119(94)90340-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The complete nucleotide sequence of the gene encoding an alkaline serine protease from Bacillus smithii has been determined. Degenerate oligodeoxyribonucleotide primers were used to prime the amplification of a 507-bp sequence of the gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction, using as template self-ligated DNA fragments. The deduced amino-acid sequence is compared to serine proteases from B. amyloliquefaciens, B. licheniformis, B. subtilis and Thermus aquaticus.

引用

页码：149 / 150

页数：2

共 7 条

[1] NUMERICAL-ANALYSIS AND DNA-BASE COMPOSITIONS OF SOME THERMOPHILIC BACILLUS SPECIES [J].