A PROTEIN COMPLEX MEDIATING MESSENGER-RNA DEGRADATION IN ESCHERICHIA-COLI

被引：204
作者：
PY, B
CAUSTON, H
MUDD, EA
HIGGINS, CF
机构：
[1] UNIV OXFORD,JOHN RADCLIFFE HOSP,INST MOLEC MED,IMPERIAL CANC RES FUND LABS,OXFORD OX3 9DU,ENGLAND
[2] UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT CLIN BIOCHEM,OXFORD OX3 9DU,ENGLAND
来源：
MOLECULAR MICROBIOLOGY | 1994年 / 14卷 / 04期
基金：
英国惠康基金;
关键词：
D O I：
10.1111/j.1365-2958.1994.tb01309.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
mRNA degradation in Escherichia coli is mediated by a combination of exo- and endoribonucleases. We present evidence for a multiprotein complex which includes at least two enzymes that play important roles in mRNA degradation: the exoribonuclease polynucleotide phosphorylase (PNPase) and the endoribonuclease RNase E. An activity which impedes the processive activity of PNPase at stem-loop structures also appears to be associated with the complex. This complex is estimated to have a molecular mass of about 500 kDa and includes several additional polypeptides whose functions are unknown. The identification of a complex which includes several activities associated with mRNA degradation has implications for the mechanisms and co-ordinated control of mRNA degradation.
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页码：717 / 729
页数：13
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