HEPG2 CELLS - AN INVITRO MODEL FOR P450-DEPENDENT METABOLISM OF ACETAMINOPHEN

被引：81
作者：
ROE, AL
SNAWDER, JE
BENSON, RW
ROBERTS, DW
CASCIANO, DA
机构：
[1] NATL CTR TOXICOL RES,DIV BIOCHEM TOXICOL,JEFFERSON,AR 72079
[2] UNIV ARKANSAS MED SCI HOSP,DEPT PHARMACOL & TOXICOL,LITTLE ROCK,AR 72206
[3] UNIV ARKANSAS MED SCI HOSP,DEPT MOLEC BIOL & BIOCHEM,LITTLE ROCK,AR 72206
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 190卷 / 01期
关键词：
D O I：
10.1006/bbrc.1993.1003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The human hepatoma cell line, HepG2, retains many cellular functions often lost by cells in culture. This research examined the constitutive bioactivation of acetaminophen and P450-dependent activity in microsomes from HepG2 cells and the effect of 0.1% acetone pretreatment on these activities. Low levels of acetaminophen bioactivation, P450 IIE1 activity, and P450 IA1- IA2 activity were demonstrate in non-induced HepG2 microsomes. Acetone increased acetaminophen bioactivation and IIE1-dependent metabolism but not P450 IA1-IA2-dependent activity. Thus, HepG2 cells may provide an in vitro model for assessing human xenobiotic metabolism of acetaminophen and other drugs. © 1993 Academic Press, Inc.
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页码：15 / 19
页数：5
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