EFFECT OF CYTOCHALASIN-B, NOCODAZOLE AND IRRADIATION ON MIGRATION AND INTERNALIZATION OF CELLS AND MICROSPHERES IN TUMOR-CELL SPHEROIDS

被引：24

作者：

DORIE, MJ ^{[1
]}

KALLMAN, RF ^{[1
]}

COYNE, MA ^{[1
]}

机构：

[1] STANFORD UNIV, MED CTR, DEPT RADIOL, DIV RADIOBIOL RES, STANFORD, CA 94305 USA

来源：

EXPERIMENTAL CELL RESEARCH | 1986年 / 166卷 / 02期

关键词：

D O I：

10.1016/0014-4827(86)90483-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The effect of cytochalasin B (CB), nocodazole, and irradiation on the adherence and internalization of 3H-labeled EMT6 spheroid-derived single cells and inert microspheres in unlabeled, intact EMT6 multicellular spheroids has been examined. CB inhibited adhesion and internal migration, whereas nocodazole did not stop adhesion but did prevent later internalization. Treatment of labeled cells with 5,15 and 25 Gy 250 kV X-rays before adherence did not effect their adherence or later internalization. The same radiation treatments administered to the spheroids either immediately before or after the introduction of unirradiated single cells did not affect adherence, but the depths reached by labeled cells and microspheres were reduced largely because of the consequent reduction in spheroid growth. Microsphere size (9, 15, or 25 .mu.m) and surface charge (negative, or nonionic) had minimal, if any, effect on the adherence and internalization of these particles.

引用

页码：370 / 378

页数：9