DIRECT SEQUENCING OF HEPATITIS-A VIRUS-STRAINS ISOLATED DURING AN EPIDEMIC IN FRANCE

被引：29

作者：

APAIREMARCHAIS, V

ROBERTSON, BH

AUBINEAUFERRE, V

LEROUX, MG

LEVEQUE, F

SCHWARTZBROD, L

BILLAUDEL, S

机构：

[1] INST BIOL,VIROL LAB,F-44035 NANTES 01,FRANCE

[2] MOLEC GENET LAB,NANTES,FRANCE

[3] CTR RECH SERV SANTE ARMEES,GRENOBLE,FRANCE

[4] FAC PHARM NANCY,DEPT VIROL,NANCY,FRANCE

[5] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,HEPATITIS BRANCH,ATLANTA,GA 30341

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 11期

关键词：

D O I：

10.1128/AEM.61.11.3977-3980.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Direct sequencing of PCR products was used to study the VP1 region of the hepatitis A virus (HAV) genome (position 2199 to 2356) of nine strains isolated from human stools collected during a hepatitis A epidemic (western France, 1992), three strains from environmental samples (1990, 1991, and 1992), and two HAV cell culture isolates (the French strain CF53/Lyon and strain CLP). These viruses differed from CF53/Lyon (genotype I) by between 1 and 10.3%, and results indicated the existence of two groups of strains belonging to two different subgenotypes (IA and IB). With this sequencing technique it was possible to monitor the epidemiology of HAV and study its relations.

引用

收藏

页码：3977 / 3980

页数：4

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