EVIDENCE THAT LOCUSTATACHYKININ-I IS INVOLVED IN RELEASE OF ADIPOKINETIC HORMONE FROM LOCUST CORPORA-CARDIACA

被引:81
作者
NASSEL, DR
PASSIER, PCCM
ELEKES, K
DIRCKSEN, H
VULLINGS, HGB
CANTERA, R
机构
[1] UNIV UTRECHT,DEPT EXPTL ZOOL,3584 CH UTRECHT,NETHERLANDS
[2] HUNGARIAN ACAD SCI,BALATON LIMNOL RES INST,DEPT EXPTL ZOOL,H-8237 TIHANY,HUNGARY
[3] UNIV BONN,DEPT ZOOPHYSIOL,D-53115 BONN,GERMANY
关键词
LOCUSTA MIGRATORIA; NEUROPEPTIDE; LOCUSTATACHYKININ; ADIPOKINETIC HORMONE; HORMONE RELEASE; CORPUS CARDIACUM;
D O I
10.1016/0167-0115(95)00043-B
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The glandular cells of the corpus cardiacum of the locust Locusta migratoria, known to synthesize and release adipokinetic hormones (AKH), are contacted by axons immunoreactive to an antiserum raised against the locust neuropeptide locustatachykinin I (LomTK I). Electron-microscopical immunocytochemistry reveals LomTK immunoreactive axon terminals, containing granular vesicles, in close contact with the glandular cells cells. Release of AKH I from isolated corpora cardiaca of the locust has been monitored in an in vitro system where the amount of AKH I released into the incubation saline is determined by reversed phase high performance liquid chromatography with fluorometric detection. We could show that LomTK I induces release of AKH from corpora cardiaca in a dose-dependent manner when tested in a range of 10-200 mu M. This is thus the first clear demonstration of a substance inducing release of AKH, correlated with the presence of the substance in fibers innervating the AKH-synthesizing glandular cells, in the insect corpora cardiaca.
引用
收藏
页码:297 / 310
页数:14
相关论文
共 45 条
[1]   CARBOHYDRATE AND FAT AS A FUEL FOR INSECT FLIGHT . A COMPARATIVE STUDY [J].
BEENAKKE.AM .
JOURNAL OF INSECT PHYSIOLOGY, 1969, 15 (03) :353-&
[2]   INSECT ADIPOKINETIC HORMONES [J].
BEENAKKERS, AMT ;
BLOEMEN, REB ;
DEVLIEGER, TA ;
VANDERHORST, DJ ;
VANMARREWIJK, WJA .
PEPTIDES, 1985, 6 :437-444
[3]   OCTOPAMINE DISTRIBUTION IN THE LOCUSTA-MIGRATORIA NERVOUS AND NON-NERVOUS, SYSTEMS [J].
DAVID, JC ;
LAFONCAZAL, M .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1979, 64 (01) :161-164
[4]  
DIEDEREN JHB, 1987, CELL TISSUE RES, V249, P379
[5]  
DIRCKSEN H, 1987, CELL TISSUE RES, V250, P377
[6]  
FISCHERLOUGHEED J, 1993, J EXP BIOL, P223
[7]   THE ADIPOKINETIC HORMONE RED PIGMENT-CONCENTRATING HORMONE PEPTIDE FAMILY - STRUCTURES, INTERRELATIONSHIPS AND FUNCTIONS [J].
GADE, G .
JOURNAL OF INSECT PHYSIOLOGY, 1990, 36 (01) :1-12
[8]   ADIPOKINETIC HORMONES - FUNCTIONS AND STRUCTURES [J].
GOLDSWORTHY, G ;
MORDUE, W .
BIOLOGICAL BULLETIN, 1989, 177 (02) :218-224
[9]   IN-VIVO STUDIES ON RELEASE OF HORMONES FROM CORPORA CARDIACA OF LOCUSTS [J].
GOLDSWORTHY, GJ ;
MORDUE, W ;
JOHNSON, RA .
JOURNAL OF COMPARATIVE PHYSIOLOGY, 1972, 79 (01) :85-+
[10]  
Hardie DG, 1991, BIOCH MESSENGERS HOR