IDENTIFICATION OF THE ORIGIN OF REPLICATION OF THE EUKARYOTE DICTYOSTELIUM-DISCOIDEUM NUCLEAR PLASMID DDP2

被引：26

作者：

CHANG, ACM

SLADE, MB

WILLIAMS, KL

机构：

[1] School of Biological Sciences, Macquarie University, Sydney

来源：

PLASMID | 1990年 / 24卷 / 03期

关键词：

D O I：

10.1016/0147-619X(90)90004-V

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Ddp2 is a 5.8-kb, high-copy-number, nuclear plasmid found in the eukaryoteDictyostelium discoideum. We have identified two functional domains, a large open reading frame (Rep gene) and a 626-bp fragment containing an origin of replication (ori). The ori, when cloned into a shuttle vector, confers stable extrachromosomal replication inD. discoideum, provided that the Rep gene, which acts intrans, is integrated into the host genome. Ddp2 carries a 501-bp imperfect inverted repeat, and part of the ori overlaps with one of these repeats. The ori sequence contains two direct repeats of 49 bp comprising two 10-bp "TGTCATGACA" palindromes separated by a poly(T · A) sequence. Deletion of either 49-bp repeat abolished extrachromosomal replication. © 1990 Academic Press, Inc. All rights reserved.

引用

页码：208 / 217

页数：10

共 31 条

[1]

AHERN KG, 1988, NUCLEIC ACIDS RES, V16, P6825

[2] ESTABLISHMENT OF THE NUCLEAR LOCATION OF DICTYOSTELIUM-DISCOIDEUM PLASMIDS [J].