A NEW METHOD FOR EXTRACTING DNA OR RNA FOR POLYMERASE CHAIN-REACTION

被引：56
作者：
YAMADA, O
MATSUMOTO, T
NAKASHIMA, M
HAGARI, S
KAMAHORA, T
UEYAMA, H
KISHI, Y
UEMURA, H
KURIMURA, T
机构：
[1] TOTTORI UNIV,SCH MED,DEPT VIROL,YONAGO,TOTTORI 683,JAPAN
[2] FUSO PHARMACEUT IND LTD,DEPT RES & DEV,OSAKA,JAPAN
来源：
JOURNAL OF VIROLOGICAL METHODS | 1990年 / 27卷 / 02期
关键词：
Glass powder; HIV; HTLV-1; PCR;
D O I：
10.1016/0166-0934(90)90136-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of glass powder suspension for the extraction of RNA or DNA was studied to simplify the procedures of polymerase chain reaction (PCR). Using this procedure, proviral DNA of human T-lymphotropic virus type-1 (HTLV-1) in the blood of an asymptomatic virus carrier and viral RNA of human immunodeficiency virus (HIV) in the blood of an AIDS patient were easily detected by PCR employing glass powder. The use of glass powder is a simple and highly efficient procedure for the extraction of DNA or RNA, and can be applied for routine PCR. © 1990.
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页码：203 / 209
页数：7
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