Identification of Salinity Stress Responsive Proteins in Vigna radiata Var. radiata (L.) Wilczek Leaves

Identification of stress responsive proteins assists in understanding of physiological mechanisms behind stress tolerance and prominently in identification of candidate genes for transgenesis that may result increased crop productivity. In the present exploration, the effect of increased salinity on Vigna radiata var. radiata (L.) Wilczek in its vegetative stage was studied. The seeds were sown in red, clayey soil in pots and watered with different concentrations of NaCl viz., 0( Control), 50, 100,150, 200mM. Proteomic analysis was made with the leaf tissues of 45 DAS plants that included extraction of proteins, protein assays, 2 Dimensional gel electrophoresis and further study on differentially expressed proteins by Peptide Mass Finger printing and MASCOT search. The analysis revealed the presence of many differentially expressed proteins including glutathione-s-transferase, kunitz type trypsin inhibitors.