EXPRESSION CLONING IN YEAST OF A CDNA-ENCODING A BROAD-SPECIFICITY AMINO-ACID PERMEASE FROM ARABIDOPSIS-THALIANA

被引：182
作者：
FROMMER, WB
HUMMEL, S
RIESMEIER, JW
机构：
[1] Institut Genbiologische Forschung, Abteilung Willmitzer, D-1000 Berlin 33
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 13期
关键词：
AMINO ACID TRANSPORT; NITROGEN PARTITIONING; PROTON SYMPORT; NEUTRAL SYSTEM;
D O I：
10.1073/pnas.90.13.5944
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To study amino acid transport in plants at the molecular level, we have isolated an amino acid permease cDNA from Arabidopsis thaliana by complementation of a yeast mutant defective in proline uptake with a cDNA. The predicted polypeptide of 53 kDa is highly hydrophobic with 12 putative membrane-spanning regions and shows no significant homologies to other known transporters. Expression of the cDNA enables the yeast mutant to take up L-[C-14]proline. Competition studies argue for a broad but stereospecific substrate recognition by the permease, which resembles neutral or general amino acid transport systems from Chlorella and higher plants. Both pH dependence and inhibition by protonophores are consistent with a proton symport mechanism.
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页码：5944 / 5948
页数：5
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