EXPRESSION OF HUMAN CHORIONIC-GONADOTROPIN BETA-SUBUNIT GENES IN SUPERFICIAL AND INVASIVE BLADDER CARCINOMAS

Increased serum levels of human chorionic gonadotropin beta subunit (hCG beta) were described previously in patients with bladder cancer. To obtain insight into such production of hCG beta, the expression of hCG beta 7, 8, 5, and 3 genes in bladder carcinomas and normal urothelia was investigated by reverse transcription PCR. Surprisingly, hCG beta mRNAs were detected in both normal urothelial and carcinomatous cells. However, tumor progression was characterized by different patterns of transcription of the hCG beta genes; the beta 7 gene was the only gene transcribed in normal urothelia and T-a tumors included in this study, whereas in addition to beta 7, genes beta 5, 8, and 3 were transcribed in T-1 to T-4 tumors. Moreover, transcription levels of the latter three genes increased with the stage of the disease. These observations show that dramatic modifications in the expression of hCG beta genes accompany progression of bladder carcinomas.