CALCIUM-DEPENDENT ACTIVATION OF SKELETAL-MUSCLE CA2+ RELEASE CHANNEL (RYANODINE RECEPTOR) BY CALMODULIN

被引：76

作者：

BURATTI, R ^{[1
]}

PRESTIPINO, G ^{[1
]}

MENEGAZZI, P ^{[1
]}

TREVES, S ^{[1
]}

ZORZATO, F ^{[1
]}

机构：

[1] UNIV FERRARA,IST PATOL GEN,I-44100 FERRARA,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 213卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2238

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study terminal cisternae vesicles from rabbit skeletal muscle were fused into planar bilayers and the effect of calmodulin on single Ca2+ release channel currents was investigated. In the presence of 10(-7) and 10(-9) M free [Ca2+], nanomolar concentrations of calmodulin activated the channel by increasing the open probability of single-channel events in a dose dependent manner. The activatory effect of calmodulin was reversed by 10 mu M ruthenium red. At 10(-5) M free [Ca2+], calmodulin (0.1-1 mu M) inhibited channel activity. Calmodulin overlays were carried out using concentrations of Ca2+ similar to those used for the planar lipid bilayer assays. In the presence of 10(-7) M [Ca2+], calmodulin bound to the ryanodine receptor, to a region defined by residues 2937-3225 and 3546-3655. These results suggest that calmodulin may activate the Ca2+-release channel (ryanodine-receptor) by interacting with binding sites localized in the central portion of the RYR protomer. (C) 1995 Academic Press, Inc.

引用

页码：1082 / 1090

页数：9

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