SERIAL ANALYSIS OF GENE-EXPRESSION

被引：3069
作者：
VELCULESCU, VE
ZHANG, L
VOGELSTEIN, B
KINZLER, KW
机构：
[1] JOHNS HOPKINS UNIV, CTR ONCOL, HOWARD HUGHES MED INST, BALTIMORE, MD 21231 USA
[2] JOHNS HOPKINS UNIV, PROGRAM HUMAN GENET & MOLEC BIOL, BALTIMORE, MD 21231 USA
来源：
SCIENCE | 1995年 / 270卷 / 5235期
关键词：
D O I：
10.1126/science.270.5235.484
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.
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收藏
页码：484 / 487
页数：4
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