TRANSFORMATION OF PROGESTERONE BY A THERMOPHILIC BACILLUS

被引：9

作者：

SMITH, KE

WILLIAMS, RAD

SIDESO, O

机构：

[1] Department of Biochemistry, Faculty of Basic Medical Science, Queen Mary and Westfield College, London

来源：

FEMS MICROBIOLOGY LETTERS | 1992年 / 92卷 / 01期

关键词：

THERMOPHILIC BACILLUS; STEROID TRANSFORMATION; 6-ALPHA-HYDROXYPROGESTERONE; 6-BETA-HYDROXYPROGESTERONE; 20-ALPHA-DIHYDROPROGESTERONE; 20-BETA-DIHYDROPROGESTERONE; CYTOCHROME-P450;

D O I：

10.1016/0378-1097(92)90537-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

When a thermophilic bacillus was incubated with progesterone for approx. 18 h at 65-degrees-C, four progesterone-based metabolites were produced in moderate quantities. Two products were found to be hydroxy derivatives, i.e. 6-alpha-hydroxyprogesterone and 6-beta-hydroxyprogesterone and two were C-20 reduced epimers of progesterone, i.e. 20-alpha- and 20-beta-dihydroprogesterone. 6-Alpha-hydroxyprogesterone is a rare microbial transformation product. Inhibition of hydroxylation by azole-based fungicides and the presence of a carbon-monoxide-reduced difference-spectrum absorbance maximum at 448 nm in the soluble cell fraction suggest that the hydroxylase(s) might be cytochrome(s) P450. The dihydroprogesterones were probably produced by oxidoreductases.

引用

页码：29 / 34

页数：6

共 17 条

[1] STUDIES ON THE SUBSTRATE-SPECIFICITY AND INDUCIBILITY OF CYTOCHROME P-450MEG [J].