SENSITIVITY ENHANCEMENT OF EVANESCENT-WAVE IMMUNOASSAY

被引：3

作者：

YOSHIDA, M

SHIGEMORI, K

SUGIMURA, M

MATANO, M

机构：

[1] Med. Electron. Dept., Daikin Ind. Ltd., Shiga

来源：

MEASUREMENT SCIENCE AND TECHNOLOGY | 1993年 / 4卷 / 10期

关键词：

D O I：

10.1088/0957-0233/4/10/008

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A fluoro-immunosensor has been developed using evanescent wave technology. Our system uses a disposable device consisting of the waveguide and the flow cell. The effect of an excitation wavelength on the sensitivity of an evanescent wave immunoassay system has been studied experimentally. Background fluorescence from the polymethylmethacrylate waveguide is reduced by shifting to a longer excitation wavelength. The system using an excitation wavelength of 650 nm is found to possess sensitivity an order of magnitude higher than that obtained in conventional systems.

引用

页码：1077 / 1079

页数：3

共 12 条

[1] REMOTE FIBER-OPTIC BIOSENSORS BASED ON EVANESCENT-EXCITED FLUORO-IMMUNOASSAY - CONCEPT AND PROGRESS [J].