STUDIES ON THE RELATIONSHIP BETWEEN CELL-PROLIFERATION AND CELL-DEATH - OPPOSITE PATTERNS OF SGP-2 AND ORNITHINE DECARBOXYLASE MESSENGER-RNA ACCUMULATION IN PHA-STIMULATED HUMAN-LYMPHOCYTES

被引：37

作者：

GRASSILLI, E ^{[1
]}

BETTUZZI, S ^{[1
]}

MONTI, D ^{[1
]}

INGLETTI, MC ^{[1
]}

FRANCESCHI, C ^{[1
]}

CORTI, A ^{[1
]}

机构：

[1] UNIV MODENA,IST PATOL GEN,I-41100 MODENA,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 180卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)81254-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To assess the relationship between cell proliferation and cell death, the mRNA accumulation of ornithine decarboxylase (ODC) and sulfated glycoprotein 2 (SGP-2) were measured in human peripheral blood lymphocytes (HPBL) 2-6 hours after stimulation with phytohemoagglutinin (PHA). ODC is the rate limiting enzyme of polyamines biosynthesis and its early induction in mitogen-stimulated lymphocytes has been reported. On the other hand, SGP-2, a glycoprotein present in most mammalian tissues, is induced in classical models of apoptosis, such as dexamethasone-treated thymocytes. Indeed, a consistent amount of SGP-2 mRNA in quiescent HPBL, an early and progressive decrease of SGP-2 mRNA and a parallel increase of ODC mRNA accumulation, were observed, in PHAstimulated HPBL, suggesting that concomitant repression of SGP-2 and induction of ODC genes contribute for the cell entering the cell cycle. © 1991 Academic Press, Inc.

引用

页码：59 / 63

页数：5

共 18 条

[1] ANTIIMMUNOGLOBULINS INDUCE DEATH BY APOPTOSIS IN WEHI-231 B-LYMPHOMA CELLS [J].