GEL-FILTRATION STUDY OF MUSHROOM PROTEINS

被引：2

作者：

MILETIC, I

STANIMIROVIC, D

STANIMIROVIC, S

机构：

[1] Institute of Bromatology, Faculty of Pharmacy, Belgrade, 11000

来源：

CHROMATOGRAPHIA | 1990年 / 29卷 / 5-6期

关键词：

Gel permeation chromatography; Mushroom proteins; Protein structures;

D O I：

10.1007/BF02317906

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins from six edible wild mushrooms were extracted by sodium chloride, ethanol or sodium hydroxide and analysed by gel filtration on Sephacryl S-200. Analysis of the whole extracts and the separated proteins of different molecular weight as well as the determination of their amino acid content demonstrated that the soluble mushroom proteins are dimers, trimers and decamers of the simple main protein (polypeptide) with an approximate molecular weight of 15,000. The polymers were stabilized by both covalent and non-covalent forces as demonstrated by gel filtration in acetic acid before and after reduction of the covalent bonds. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：214 / 216

页数：3