DETERMINATION OF UREA IN BLOOD-SERUM BY A UREASE BIOSENSOR BASED ON AN ION-SENSITIVE FIELD-EFFECT TRANSISTOR

被引：51

作者：

BOUBRIAK, OA

SOLDATKIN, AP

STARODUB, NF

SANDROVSKY, AK

ELSKAYA, AK

机构：

[1] INST MOLEC BIOL & GENET,150 ZABOLOTNOGO ST,KIEV 252143,UKRAINE

[2] KIEV TG SHEVCHENKO STATE UNIV,KIEV 252017,UKRAINE

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1995年 / 27卷 / 1-3期

关键词：

BIOSENSORS; BLOOD SERUM; ISFETS; UREA;

D O I：

10.1016/0925-4005(94)01633-S

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A urease biosensor for the determination of urea in solutions and blood serum is made by immobilising urease in a bovine serum albumin membrane on the surface of an ion-sensitive field-effect transistor (ISFET). The sensor gives a linear response for concentrations of urea up to 2 mM at optimum parameters of the working buffer (10 mM potassium buffer, pH 7.4 with 200 mM NaCl). The high level of proteins in the serum decreases the biosensor response, but a 25-fold serum dilution gives reliable measurements. Twenty analyses can be made without a change in the sensor output. Repeated values do not differ by more than 10%. Urea measurements with the biosensor and the chemical urease-indophenol method show a high correlation. We propose that this biosensor is suitable for the medical determination of urea in blood serum.

引用

页码：429 / 431

页数：3

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