EFFECT OF GROWTH ENVIRONMENT ON THE STABILITY OF A RECOMBINANT SHUTTLE PLASMID, PCPPS-31, IN ESCHERICHIA-COLI

被引：25
作者：
GUPTA, R
SHARMA, P
VYAS, VV
机构：
[1] INST MICROBIAL TECHNOL, CTR BIOCHEM ENGN RES & PROC DEV, CHANDIGARH 160014, INDIA
[2] PUNJAB UNIV, DEPT MICROBIOL, CHANDIGARH 160014, INDIA
来源：
JOURNAL OF BIOTECHNOLOGY | 1995年 / 41卷 / 01期
关键词：
PLASMID STABILITY; GROWTH RATE; ESCHERICHIA-COLI; ENVIRONMENT;
D O I：
10.1016/0168-1656(95)00049-V
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effect of environmental growth conditions on the segregational stability of a recombinant Escherichia coli-Bacillus subtilis shuttle plasmid pCPPS-31 which expresses carboxymethyl cellulase (CMCase) and contains the neomycin resistance gene (Ne-r), was studied in E. coli DH5 alpha. The stability increased with the decrease in medium complexity. Lowering culture temperatures had a negative effect on stability. Stability was maximum at 100 rpm and a medium/flask volume ratio of 1:10. A pH range of 5-8 had no significant effect on stability. The recombination (rec) and gyrase (gyr) backgrounds of E. coli hosts had no apparent effect on plasmid stability. The plasmid was structurally stable under all circumstances.
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页码：29 / 37
页数：9
相关论文
共 45 条
[1]   EFFECTS OF TEMPERATURE ON PLASMID STABILITY AND PENICILLINASE PRODUCTIVITY IN A TRANSFORMANT OF BACILLUS-STEAROTHERMOPHILUS [J].
AIBA, S ;
KOIZUMI, J .
BIOTECHNOLOGY AND BIOENGINEERING, 1984, 26 (09) :1026-1031
[2]   ON THE FORMATION OF SPONTANEOUS DELETIONS - THE IMPORTANCE OF SHORT SEQUENCE HOMOLOGIES IN THE GENERATION OF LARGE DELETIONS [J].
ALBERTINI, AM ;
HOFER, M ;
CALOS, MP ;
MILLER, JH .
CELL, 1982, 29 (02) :319-328
[3]   GYRASE-DEPENDENT STABILIZATION OF PSC101 PLASMID INHERITANCE BY TRANSCRIPTIONALLY ACTIVE PROMOTERS [J].
BEAUCAGE, SL ;
MILLER, CA ;
COHEN, SN .
EMBO JOURNAL, 1991, 10 (09) :2583-2588
[4]   STABILITY IN CONTINUOUS CULTURES OF RECOMBINANT BACTERIA - A METABOLIC APPROACH [J].
BENTLEY, WE ;
KOMPALA, DS .
BIOTECHNOLOGY LETTERS, 1990, 12 (05) :329-334
[5]   PBR322-DERIVED MULTICOPY PLASMIDS HARBORING LARGE INSERTS ARE OFTEN DIMERS IN ESCHERICHIA-COLI K-12 [J].
BERG, CM ;
LIU, L ;
COON, M ;
STRAUSBAUGH, LD ;
GRAY, P ;
VARTAK, NB ;
BROWN, M ;
TALBOT, D ;
BERG, DE .
PLASMID, 1989, 21 (02) :138-141
[6]   EFFECT OF GROWTH-RATE ON PLASMID MAINTENANCE BY ESCHERICHIA-COLI HB101(PAT153) [J].
BROWNLIE, L ;
STEPHENSON, JR ;
COLE, JA .
JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 :2471-2480
[7]  
CAULCOTT CA, 1987, J GEN MICROBIOL, V133, P1881
[8]   THE EFFECT OF OXYGEN LIMITATION ON STABILITY OF A RECOMBINANT PLASMID IN SACCHAROMYCES-CEREVISIAE [J].
CAUNT, P ;
IMPOOLSUP, A ;
GREENFIELD, PF .
BIOTECHNOLOGY LETTERS, 1989, 11 (01) :5-10
[9]  
CHEW LCK, 1988, FEMS MICROBIOL LETT, V56, P101
[10]  
EHRLICH SD, 1986, BACILLUS MOL GENETIC, P27
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