TRANSMITTER-MEDIATED INHIBITION OF N-TYPE CALCIUM CHANNELS IN SENSORY NEURONS INVOLVES MULTIPLE GTP-BINDING PROTEINS AND SUBUNITS

被引:121
作者
DIVERSEPIERLUISSI, M
GOLDSMITH, PK
DUNLAP, K
机构
[1] TUFTS UNIV,SCH MED,DEPT NEUROSCI,BOSTON,MA 02111
[2] NIDDKD,METAB DIS BRANCH,BETHESDA,MD 20892
关键词
D O I
10.1016/0896-6273(95)90254-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The modulation of voltage-activated Ca2+ channels by neurotransmitters and peptides is very likely a primary means of regulating Ca2+-dependent physiological functions such as neurosecretion, muscle contraction, and membrane excitability. In neurons, N-type Ca2+ channels (defined as omega-conotoxin GVIA-sensitive) are one prominent target for transmitter-mediated inhibition. This inhibition is widely thought to result from a shift in the voltage dependence of channel gating. Recently, however, voltage-independent inhibition has also been described for N channels. As embryonic chick dorsal root ganglion neurons express both of these biophysically distinct modulatory pathways, we have utilized these cells to test the hypothesis that the voltage-dependent and -independent actions of transmitters are mediated by separate biochemical pathways. We have confirmed this hypothesis by demonstrating that the two modulatory mechanisms activated by a single transmitter involve not only different classes of G protein but also different G protein subunits.
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页码:191 / 200
页数:10
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