A PHOTO-REACTIVE DERIVATIVE OF GANGLIOSIDE GM1 SPECIFICALLY CROSS-LINKS VIP21-CAVEOLIN ON THE CELL-SURFACE

被引：161

作者：

FRA, AM

MASSERINI, M

PALESTINI, P

SONNINO, S

SIMONS, K

机构：

[1] UNIV MILAN, DEPT MED CHEM & BIOCHEM, I-20133 MILAN, ITALY

[2] EUROPEAN MOLEC BIOL LAB, D-69117 HEIDELBERG, GERMANY

来源：

FEBS LETTERS | 1995年 / 375卷 / 1-2期

关键词：

CAVEOLAE; CROSS-LINKING; GANGLIOSIDE GM1; SPHINGOLIPID; VIP21-CAVEOLIN;

D O I：

10.1016/0014-5793(95)95228-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have shown that sphingolipids mag be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radio-labeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain, After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae, This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae.

引用

页码：11 / 14

页数：4

共 33 条

[1] 3-DIMENSIONAL STRUCTURE OF THE OLIGOSACCHARIDE CHAIN OF GM1 GANGLIOSIDE REVEALED BY A DISTANCE-MAPPING PROCEDURE - A ROTATING AND LABORATORY FRAME NUCLEAR OVERHAUSER ENHANCEMENT INVESTIGATION OF NATIVE GLYCOLIPID IN DIMETHYL-SULFOXIDE AND IN WATER DODECYLPHOSPHOCHOLINE SOLUTIONS [J].