PERSISTENT DECREASE IN SPERMIDINE/SPERMINE N1-ACETYLTRANSFERASE ACTIVITY IN POST-REGENERATION RAT-LIVER

The activity of cytosolic spermidine/ spermine N 1-acetyltransferase and polyamine levels were measured in livers of rats killed at different times after partial hepatectomy. The enzyme activity, which showed an early increase a few hours after the operation, had significantly and persistently decreased by the time the liver mass had been regained (10 days) compared to intact rats or sham-operated rats, and up to 30 days after partial hepatectomy, compared to intact rats of the same age. The early induction of spermidine/ spermine N1-acetyltransferase activity was also confirmed by the high level of N1-acetylspermidine, which normally is undetectable, and by a dramatic increase in putrescine content. Spermidine levels were increased during the first 10 days after partial hepatectomy, i.e., when the liver had regained its mass, and progressively decreased after this time until 30 days after the operation. The changes in spermine content were similar but not as marked as the changes in spermidine content at the different observation times.