COMPARATIVE H-2-NMR AND P-31-NMR STUDY ON THE PROPERTIES OF PALMITOYLLYSOPHOSPHATIDYLCHOLINE IN BILAYERS WITH GRAMICIDIN, CHOLESTEROL AND DIPALMITOYLPHOSPHATIDYLCHOLINE

被引:45
作者
KILLIAN, JA
BORLE, F
DEKRUIJFF, B
SEELIG, J
机构
[1] STATE UNIV UTRECHT, DEPT BIOCHEM, PADUALAAN 8, TRANSITORIUM 3, 3584 CH UTRECHT, NETHERLANDS
[2] UNIV BASEL, BIOCTR, DEPT BIOPHYS CHEM, CH-4056 BASEL, SWITZERLAND
[3] STATE UNIV UTRECHT, INST MOLEC BIOL, 3584 CH UTRECHT, NETHERLANDS
关键词
D O I
10.1016/0005-2736(86)90073-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The stoichiomeric palmitoyllysophosphatidylcholine (lysoPC)/gramicidin (4:1, mol/mol) lamellar complex (Killian, J.A., De Kruijff, B., Van Echteld, C.J.A., Verkleij, A.J., Leunissen-Bijvelt, J. and De Gier, J. (1983) Biochem. Biophys. Acta 728, 141-144) is a useful model system to investigate the various aspects of lipid protein interactions. To study the effect of gramicidin on local order and motion of 1-palmitoyl-sn-glycero-3-phosphocholine (lysoPC) we employed 31P and 2H nuclear magnetic resonance (NMR) using selectively deuterated lysoPC''s and we compared the results to those obtained for lysoPC in bilayers with cholesterol (1:1, mol/mol) and dipalmitoylphosphatidylcholine (DPPC) (1:4, mol/mol). 2H-NMR experiments on acyl chain deuterated lysoPC showed similar quadrupole splittings in the liquid crystalline state for the lysoPC/DPPC and the lysoPC/gramicidin samples. In the lysoPC/cholesterol sample an increase of the quadrupole splitting was found. T1 measurements showed that gramicidin decreases the lysoPC acyl chain motion, especially at the C12 position. In the lysoPC/cholesterol sample an increase of motion was observed as compared to lysoPC in fluid bilayers of DPPC. 31P-NMR and 2-H-NMR measurements of lysoPC, deuterated at the .alpha.- and .beta.-position of the choline moiety, indicated an increase in headgroup flexibility in all samples as compared to the parent compound DPPC. In addition, a change in headgroup conformation was observed. The .alpha.- and .beta.-segments in all samples exhibited concerted motion. It was found that also in the polar headgroup gramicidin induces a decrease of the rate of motion.
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页码:133 / 142
页数:10
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