CONSTRUCTION OF SITE-SPECIFIC MUTANTS OF E5A PROTEIN OF HPV-11 USING THE POLYMERASE CHAIN-REACTION AND A SINGLE MUTANT PRIMER

被引：7

作者：

CHEN, SL ^{[1
]}

HAN, CP ^{[1
]}

TSAO, YP ^{[1
]}

机构：

[1] NATL DEF MED CTR,GRAD INST MED SCI,TAIPEI,TAIWAN

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 42卷 / 01期

关键词：

HUMAN PAPILLOMAVIRUS; E5A PROTEIN; POLYMERASE CHAIN REACTION; MUTANT PRIMER;

D O I：

10.1016/0166-0934(93)90179-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The method of Perrin and Gilliland (1990) was modified to create site-specific mutants. The polymerase chain reaction and a single mutant primer are needed to carry out site-specific mutagenesis. Using this method, removal of the excess primers and nucleotides from the initial amplification is not necessary. This method provides a simpler way to generate site-specific mutants.

引用

页码：89 / 94

页数：6

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