IN-VITRO FERTILIZATION OF BOVINE OOCYTES EXPOSED TO BOVINE HERPESVIRUS-1 (BHV-1)

被引：27

作者：

BIELANSKI, A

DUBUC, C

机构：

[1] Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario

来源：

REPRODUCTION IN DOMESTIC ANIMALS | 1993年 / 28卷 / 06期

关键词：

EMBRYO; IN-VITRO FERTILIZATION; BOVINE HERPESVIRUS-1;

D O I：

10.1111/j.1439-0531.1993.tb00998.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Cumulus-oocyte complexes were matured for 24 hours in the presence or absence of bovine herpes virus-1 (BHV-1, 10(6) TCID 50/ml), fertilized in vitro and then cocultured on oviductal cells to the blastocyst stage. The percentages of oocytes which cleaved were 48% (n = 905) for oocytes matured in the presence of BHV-1, and 51% (n = 1004) for the control oocytes. Comparing BHV-1-treated to control, the percentages of cleaved oocytes which developed to the blastocyst stage were 29% and 31%, respectively (p > 0.05). Embryos produced from the experimental group of oocytes tested positive for BHV-1.

引用

页码：285 / 288

页数：4

共 10 条

[1] THE INVITRO EXPOSURE TO BOVINE-RHINOTRACHEITIS VIRUS OF ZONA PELLUCIDA-MICROMANIPULATED BOVINE EMBRYOS WITH THE ZONA-PELLUCIDA DAMAGED OR REMOVED [J].