A CLUSTER OF 3 GENES (DAPA, ORF2, AND DAPB) OF BREVIBACTERIUM-LACTOFERMENTUM ENCODES DIHYDRODIPICOLINATE SYNTHASE, DIHYDRODIPICOLINATE REDUCTASE, AND A 3RD POLYPEPTIDE OF UNKNOWN FUNCTION

被引：24

作者：

PISABARRO, A ^{[1
]}

MALUMBRES, M ^{[1
]}

MATEOS, LM ^{[1
]}

OGUIZA, JA ^{[1
]}

MARTIN, JF ^{[1
]}

机构：

[1] UNIV LEON,FAC BIOL,DEPT ECOL GENET & MICROBIOL,AREA MICROBIOL,E-24071 LEON,SPAIN

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 09期

关键词：

D O I：

10.1128/JB.175.9.2743-2749.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The dapA and dapB genes, encoding, respectively, dihydrodipicolinate synthase and dihydrodipicolinate reductase, the two first enzymes of the lysine branch of the aspartic amino acid family, were cloned from the DNA of the amino acid-producing bacterium Brevibacterium lactofermentum. The two genes were clustered in a 3.5-kb Sau3AI-BamHI fragment but were separated by an open reading frame of 750 nucleotides. The protein encoded by this open reading frame had little similarity to any protein in the data banks, and its function remains unknown. The three genes were translated in Escherichia coli, giving the corresponding polypeptides.

引用

页码：2743 / 2749

页数：7

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