Enhancing catalytic activity of lipases from Candida cylindracea and Pancreatic porc for glucose laurate synthesis

Sugar esters are widely used nonionic and nontoxic bio-surfactants. Their enzymatic synthesis has important advantages over the conventional chemical synthesis. This article is concerned with modified lipases catalyzed synthesis of glucose fatty acid ester in organic solvent. Sorbitan monostearate and porous celite were used as support to modify lipases from Candida cylindracea (CCL) and Pancreatic pore (PPL). The biocatalysts prepared were tested in the synthesis of 6-O-Dodecanoyl-D-Glucopyranose by esterification of glucose and lauric acid in ethylmethylketone medium. The effects associated with the specific nature of the lipase catalyst used are discussed. Optimal conversion was around 45%, with surfactant coated PPL and with immobilized PPL and CCL after 72 hours of reaction.