IMAGING CYTOSKELETON MITOCHONDRIAL-MEMBRANE ATTACHMENTS BY EMBEDMENT-FREE ELECTRON-MICROSCOPY OF SAPONIN-EXTRACTED CELLS

被引：50

作者：

LIN, A ^{[1
]}

KROCKMALNIC, G ^{[1
]}

PENMAN, S ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 21期

关键词：

Resinless sections; Scanning electron microscopy; Stereoscopic electron microscopy;

D O I：

10.1073/pnas.87.21.8565

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Embedment-free electron microscopy images the cytoskeleton and nuclear matrix, which are very difficult to visualize in conventional electron micrographs. However, to be effective, cell structures must be depleted of soluble proteins, which otherwise shroud cell architecture. Nonionic detergents effect this extraction, releasing soluble proteins but also destroying all membranes. Saponin can permeabilize plasma membranes, releasing soluble proteins while preserving many cytoplasmic membranes. Stereoscopic electron microscopy of resinless sections shows the many connections of the cytoskeleton to mitochondrial membranes.

引用

页码：8565 / 8569

页数：5

共 24 条

[1] ACTION OF SAPONIN ON BIOLOGICAL CELL MEMBRANES [J].