Direct database searching with MALDI-PSD spectra of peptides

被引：77
作者：
Griffin, PR
MacCoss, MJ
Eng, JK
Blevins, RA
Aaronson, JS
Yates, JR
机构：
[1] MERCK RES LABS, DEPT BIOINFORMAT, RAHWAY, NJ 07065 USA
[2] UNIV WASHINGTON, DEPT MOLEC BIOTECHNOL, SEATTLE, WA 98195 USA
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 15期
关键词：
D O I：
10.1002/rcm.1290091515
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The analysis of matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) mass spectra of peptides by using the cross-correlation method for database searching is illustrated. MALDI-PSD mass spectra are shown to contain sufficient fragmentation information to uniquely identify the correct amino acid sequence from large protein databases (similar to 160 000 entries), A search employing the MALDI-PSD mass spectrum of a phosphorylated peptide that correctly identifies the amino acid sequence and the site of phosphorylation is also illustrated.
引用
收藏
页码：1546 / 1551
页数：6
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