A (1-]4)-BETA-D-GLUCURONAN EXCRETED BY A MUTANT OF THE RHIZOBIUM-MELILOTI M5N1 STRAIN

被引：33
作者：
COURTOIS, J
SEGUIN, JP
DECLOMESNIL, S
HEYRAUD, A
COLINMOREL, P
DANTAS, L
BARBOTIN, JN
COURTOIS, B
机构：
[1] UNIV PICARDIE, FAC SCI, GENIE CELLULAIRE LAB, F-80039 AMIENS, FRANCE
[2] CNRS, CTR RECH MACROMOLEC VEGETALES, F-38041 GRENOBLE, FRANCE
来源：
JOURNAL OF CARBOHYDRATE CHEMISTRY | 1993年 / 12卷 / 4-5期
关键词：
D O I：
10.1080/07328309308019399
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A mutant of the R. meliloti M5N1 strain has been selected. This strain, R. meliloti M5N1 CS (NCIMB 40472), excretes an extracellular material composed of 2-O-Ac-beta-GlcpA, 3-O-Ac-beta-GlcpA, 2,3-di-O-Ac-beta-GlcpA and three species of beta-GlcpA residues 1-->4 linked. For the culture conditions used, the weight average molecular weight of the polymer varied in the range of 6 x 10(4) < MwBAR < 4 x 10(5). High molecular weight glucuronate forms thermoreversible gels at 5 g L-1. In the presence of divalent cation such as Ca2+ or trivalent cations such as Cr3+ or Fe3+, Cross linking of the polymer occurs. This polysaccharide is the first exocellular (1-->4)-beta-D-glucuronan produced by a R. meliloti strain.
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页码：441 / 448
页数：8
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