Chronic Exposure to Particulate Nickel Induces Neoplastic Transformation in Human Lung Epithelial Cells

被引：2

作者：

Holmes, Amie L. ^{[1
,2
,3
]}

The, Therry ^{[1
,2
]}

Thompson, Kelsey ^{[1
,2
]}

Mason, Michael ^{[4
]}

Kandpal, Sanjeev ^{[4
]}

Zheng, Tongzhang ^{[5
]}

Wise, John Pierce ^{[1
,2
,3
]}

机构：

[1] Univ Southern Maine, Wise Lab Environm & Genet Toxicol, Portland, ME 04104 USA

[2] Univ Southern Maine, Maine Ctr Toxicol & Environm Hlth, Portland, ME 04104 USA

[3] Univ Southern Maine, Dept Appl Sci Med, Portland, ME 04104 USA

[4] Univ Maine, Dept Chem & Biol Engn, Orono, ME 04469 USA

[5] Yale Sch Publ Hlth, Dept Environm Hlth Sci, New Haven, CT 06520 USA

来源：

TOXICS | 2013年 / 1卷 / 01期

关键词：

particulate nickel; nickel subsulfide; chromosome damage; transformation;

D O I：

10.3390/toxics1010046

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Nickel is a well-known human lung carcinogen with the particulate form being the most potent; however, the carcinogenic mechanism remains largely unknown. Few studies have investigated the genotoxicity and carcinogenicity of nickel in its target cell, human bronchial epithelial cells. Thus, the goal of this study was to investigate the effects of particulate nickel in human lung epithelial cells. We found that nickel subsulfide induced concentration-and time-dependent increases in both cytotoxicity and genotoxicity in human lung epithelial cells (BEP2D). Chronic exposure to nickel subsulfide readily induced cellular transformation, inducing 2.55, 2.9 and 2.35 foci per dish after exposure to 1, 2.5 and 5 mu g/cm(2) nickel subsulfide, respectively. Sixty-one, 100 and 70 percent of the foci isolated from 1, 2.5, and 5 mu g/cm(2) nickel subsulfide treatments formed colonies in soft agar and the degree of soft agar colony growth increased in a concentration-dependent manner. Thus, chronic exposure to particulate nickel induces genotoxicity and cellular transformation in human lung epithelial cells.

引用

页码：46 / 59

页数：14

共 33 条

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