MONOMOLECULAR ENZYME FILMS STABILIZED BY AMPHIPHILIC POLYELECTROLYTES FOR BIOSENSOR DEVICES

被引:16
作者
EREMENKO, A
KUROCHKIN, I
CHERNOV, S
BARMIN, A
YAROSLAVOV, A
MOSKVITINA, T
机构
[1] MOSCOW MV LOMONOSOV STATE UNIV,DEPT CHEM,MOSCOW 119899,RUSSIA
[2] INST BIOL & MED CHEM,BIOGEN AMINES LAB,MOSCOW 119839,RUSSIA
关键词
LANGMUIR-BLODGETT FILMS; POLYMERS; SENSORS;
D O I
10.1016/0040-6090(94)06403-2
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
This paper describes the use of new amphiphilic polyelectrolytes for protein immobilization. Monomolecular films of glucose oxidase (GO) and monoamine oxidase (MAO) stabilized by amphiphilic polyelectrolytes (polyethyleneimine and poly-4-vinylpyridine derivatives modified by lauryl chains) were formed on a water surface in a Langmuir-Blodgett trough. The compressed films containing the enzymes were transferred onto the surface of a polypropylene membrane of a Clark electrode according to the Languir-Schaefer method. The analytical responses of the resulting biosensors were linear over the range 1-10 mM of glucose and 8-100 mu M of tyramine. Furthermore, direct functional coupling of GO and ferrocenecarboxylic acid in multilayer films stabilized by amphiphilic polyelectrolytes was demonstrated. The amperometric response of such a sensor was linear over the range 1-20 mM of glucose. The dependence of the kinetic parameters of the enzymes on the amphiphilic polyelectrolyte structures is discussed.
引用
收藏
页码:212 / 216
页数:5
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