ISOLATION OF A COMPLEMENTARY-DNA THAT ENCODES THE MAMMALIAN SPLICING FACTOR SC35

被引：222
作者：
FU, XD
MANIATIS, T
机构：
[1] Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138
来源：
SCIENCE | 1992年 / 256卷 / 5056期
关键词：
D O I：
10.1126/science.1373910
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The mammalian splicing factor SC35 is required for the first step in the splicing reaction and for spliceosome assembly. The cloning and characterization of a complementary DNA encoding this protein revealed that it is a member of a family of splicing factors that includes mammalian SF2/ASF. This family of proteins is characterized by the presence of a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal serine-arginine-rich (SR) domain. A search of the DNA sequence database revealed that the thymus-specific exon (E(T)) of the c-myb proto-oncogene is encoded on the antisense strand of the SC35 gene.
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页码：535 / 538
页数：4
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