A RELIABLE METHOD FOR RANDOM MUTAGENESIS - THE GENERATION OF MUTANT LIBRARIES USING SPIKED OLIGODEOXYRIBONUCLEOTIDE PRIMERS

被引：103

作者：

HERMES, JD ^{[1
]}

PAREKH, SM ^{[1
]}

BLACKLOW, SC ^{[1
]}

KOSTER, H ^{[1
]}

KNOWLES, JR ^{[1
]}

机构：

[1] MILLIGEN BIOSEARCH,BURLINGTON,MA 01803

来源：

GENE | 1989年 / 84卷 / 01期

关键词：

D O I：

10.1016/0378-1119(89)90148-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

引用

页码：143 / 151

页数：9

共 30 条

[11] A NEW METHOD FOR RANDOM MUTAGENESIS OF COMPLETE GENES - ENZYMATIC GENERATION OF MUTANT LIBRARIES INVITRO [J].

LEHTOVAARA, PM ;

KOIVULA, AK ;

BAMFORD, J ;

KNOWLES, JKC .

PROTEIN ENGINEERING, 1988, 2 (01) :63-68

[12] TARGETED RANDOM MUTAGENESIS - THE USE OF AMBIGUOUSLY SYNTHESIZED OLIGONUCLEOTIDES TO MUTAGENIZE SEQUENCES IMMEDIATELY 5' OF AN ATG INITIATION CODON [J].

MATTEUCCI, MD ;

HEYNEKER, HL .

NUCLEIC ACIDS RESEARCH, 1983, 11 (10) :3113-3121

[13] SACCHAROMYCES-CERVISIAE CYC1 MESSENGER-RNA 5'-END POSITIONING - ANALYSIS BY INVITRO MUTAGENESIS, USING SYNTHETIC DUPLEXES WITH RANDOM MISMATCH BASE-PAIRS [J].

MCNEIL, JB ;

SMITH, M .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3545-3551

[14]

Miller J., 1972, EXPT MOL GENETICS, P1

[15] A GENERAL-METHOD FOR SATURATION MUTAGENESIS OF CLONED DNA FRAGMENTS [J].

MYERS, RM ;

LERMAN, LS ;

MANIATIS, T .

SCIENCE, 1985, 229 (4710) :242-247

[16] A SIMPLE AND EFFICIENT PROCEDURE FOR GENERATING RANDOM POINT MUTATIONS AND FOR CODON REPLACEMENTS USING MIXED OLIGODEOXYNUCLEOTIDES [J].

NER, SS ;

GOODIN, DB ;

SMITH, M .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1988, 7 (02) :127-134

[17] CLONING OF RANDOM-SEQUENCE OLIGODEOXYNUCLEOTIDES [J].

OLIPHANT, AR ;

NUSSBAUM, AL ;

STRUHL, K .

GENE, 1986, 44 (2-3) :177-183

[18] PH-DEPENDENCE OF TRIOSE PHOSPHATE ISOMERASE REACTION [J].

PLAUT, B ;

KNOWLES, JR .

BIOCHEMICAL JOURNAL, 1972, 129 (02) :311-&

[19] COMBINATORIAL CASSETTE MUTAGENESIS AS A PROBE OF THE INFORMATIONAL CONTENT OF PROTEIN SEQUENCES [J].

REIDHAAROLSON, JF ;

SAUER, RT .

SCIENCE, 1988, 241 (4861) :53-57

[20] GAP MISREPAIR MUTAGENESIS - EFFICIENT SITE-DIRECTED INDUCTION OF TRANSITION, TRANSVERSION, AND FRAMESHIFT MUTATIONS INVITRO [J].

SHORTLE, D ;

GRISAFI, P ;

BENKOVIC, SJ ;

BOTSTEIN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (05) :1588-1592

← 1 2 3 →