Purification and Characterization of a Liver Chitinase from Golden Cuttlefish, Sepia esculenta

被引：3

作者：

Nishino, Ryo ^{[1
]}

Suyama, Akiyoshi ^{[1
]}

Ikeda, Mana ^{[1
]}

Kakizaki, Hiromi ^{[1
]}

Matsumiya, Masahiro ^{[1
]}

机构：

[1] Nihon Univ, Dept Marine Sci & Resources, Coll Bioresource Sci, Fulisawa, Kanagawa 2520880, Japan

来源：

JOURNAL OF CHITIN AND CHITOSAN SCIENCE | 2014年 / 2卷 / 03期

关键词：

Chitinase; Purification; Characterization; Liver; Sepia esculenta;

D O I：

10.1166/jcc.2014.1065

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

A chitinase was purified from the liver of the golden cuttlefish, Sepia esculenta by ammonium sulfate fractionation and column chromatography on Chitin EX, DEAE-Toyopearl 650S, and hydroxyapatite columns. The purified enzyme (SeChi) was identified as a single protein band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with an apparent molecular mass of 62 kDa. When pNp-(GlcNAc) 2 was used as a substrate, the optimum pH and optimum temperature for SeChi activity were 3.5 and 50 degrees C, respectively. The activity of SeChi was increased to 164% in the presence of 0.5 M NaCl. Of the insoluble polymer substrates, SeChi exhibited the highest activity on colloidal chitin.

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页码：238 / 243

页数：6

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