A NEW QUALITATIVE METHOD FOR DETECTING IGD IN UNCONCENTRATED CEREBROSPINAL-FLUID

被引：3

作者：

MAVRA, M

LUXTON, R

KEIR, G

MILETIC, V

THOMPSON, EJ

机构：

[1] Department of Special Chemical Pathology, The National Hospital for Neurology and Neurosurgery, London

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 144卷 / 01期

关键词：

IGD; ISOELECTRIC FOCUSING; CEREBROSPINAL FLUID;

D O I：

10.1016/0022-1759(91)90231-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a method which is capable of demonstrating the isoelectric focusing (IEF) pattern of immunoglobulin D in unconcentrated cerebrospinal fluid (CSF) samples containing as little as 0.1-0.5 ng of total IgD. The method used was an immuno-sandwich technique, with alkaline phosphatase enzyme amplification. Oligoclonal and polyclonal IgD patterns were seen in CSF samples. No cross-reactivity with other immunoglobulins (IgG, IgA and IgM) was detected.

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收藏

页码：63 / 67

页数：5

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