IDENTIFICATION AND CLONING OF A 2ND PHYTASE GENE (PHYB) FROM ASPERGILLUS-NIGER (FICUUM)

被引：48
作者：
EHRLICH, KC
MONTALBANO, BG
MULLANEY, EJ
DISCHINGER, HC
ULLAH, AHJ
机构：
[1] Southern Regional Research Center, United States Department of Agriculture, New Orleans
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 195卷 / 01期
关键词：
D O I：
10.1006/bbrc.1993.2008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An Aspergillus niger (ficuum) genomic DNA λEMBL3 library was probed with a 354-bp DNA fragment obtained by polymerase chain reaction of A. niger DNA with oligonucleotides based on partial amino acid sequence of a pH 2.5 optimum acid phosphatase. A clone containing a 1605 bp segment (phyB) encoding the 479 amino acid enzyme was isolated and found to contain four exons. Global alignment revealed 23.5% homology to Aspergillus niger phytase (PhyA); four regions of extensive homology were identified. Some of these regions may contain catalytic sites for phosphatase function. © 1993 Academic Press, Inc.
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页码：53 / 57
页数：5
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