POLY(ETHYLENIMINE)-REINFORCED LIQUID-CORE CAPSULES FOR THE CULTIVATION OF HYBRIDOMA CELLS

被引：10

作者：

HSU, YL ^{[1
]}

CHU, IM ^{[1
]}

机构：

[1] NATL TSING HUA UNIV,DEPT CHEM ENGN,HSINCHU 30043,TAIWAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 40卷 / 11期

关键词：

CELL CULTURES; ENCAPSULATION; POLY(ETHYLENIMINE);

D O I：

10.1002/bit.260401103

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The mechanical stability of liquid-core alginate-poly-L-lysine (PLL) capsules used for encapsulation of hybridoma cells can be greatly enhanced by the inclusion of poly(ethylenimine) (PEI) in the hardening solution containing calcium chloride. The PEI can also reinforce the PLL-coated carboxymethyl-celluose liquid-core capsules. The cultivation of murein hybridoma CT04 in these two capsules was carried out. Cell concentrations higher than 10(8) cells/mL per capsule were obtained with ca. 80% of the specific antibody productivity as the freely suspended cells. These capsules could withstand severe agitation and aeration in an air-lift reactor over a period of 3 weeks with minimal damage.

引用

页码：1300 / 1308

页数：9