CLONING AND SEQUENCE-ANALYSIS OF THE HUMAN GENE ENCODING EOSINOPHIL MAJOR BASIC-PROTEIN

被引：33

作者：

BARKER, RL

LOEGERING, DA

ARAKAWA, KC

PEASE, LR

GLEICH, GJ

机构：

[1] MAYO CLIN & MAYO GRAD SCH MED, DEPT IMMUNOL, ALLERG DIS RES LAB, ROCHESTER, MN 55905 USA

[2] MAYO CLIN & MAYO FDN, ROCHESTER, MN 55905 USA

[3] MAYO CLIN & MAYO GRAD SCH MED, DEPT MED, ROCHESTER, MN 55905 USA

来源：

GENE | 1990年 / 86卷 / 02期

关键词：

Allergy; Alu repeat; gene polymorphism; hypersensitivity; pro-protein; promoter; recombinant DNA; toxin;

D O I：

10.1016/0378-1119(90)90292-Y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Eosinophil granule major basic protein (MBP), a potent toxin for helminths and mammalian cells, is a single polypeptide rich in arginine. The gene, mbp, was cloned and its nucleotide sequence determined. The 3.3-kb gene consists of six exons and five introns, one of which contains an Alu family repeat. The combined exon sequence is similar to previously reported mbp cDNA sequences. The gene is immediately preceded by a putative promoter containing typical TATA and CCAAT boxes. Southern blots indicate that mbp exhibits limited polymorphism. © 1990.

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页码：285 / 289

页数：5

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