CAPILLARY ELECTROPHORESIS-BASED IMMUNOASSAY FOR CORTISOL IN SERUM

被引：99

作者：

SCHMALZING, D ^{[1
]}

NASHABEH, W ^{[1
]}

YAO, XW ^{[1
]}

MHATRE, R ^{[1
]}

REGNIER, FE ^{[1
]}

AFEYAN, NB ^{[1
]}

FUCHS, M ^{[1
]}

机构：

[1] PERSEPT BIOSYST,CAMBRIDGE,MA 02139

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 03期

关键词：

D O I：

10.1021/ac00099a019

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A competitive immunoassay for cortisol based on capillary electrophoresis (CE) and laser-induced fluorescence is described. The work involved the production of assay reagents and the development of separation conditions allowing for routine analysis of serum samples. Fluorescein-labeled cortisol was synthesized and purified. Fab fragments were produced from mouse monoclonal anticortisol antibody and purified using a POROS cation exchange chromatography column. After incubation of these reagents with serum, free and bound labeled antigen were separated by CE with high reproducibility. No prior sample cleanup of the serum samples was necessary. Serum calibration curves were established and used for the quantitation of cortisol in serum. The results demonstrate feasibility for a cortisol assay based on CE operating directly on serum samples.

引用

页码：606 / 612

页数：7