USE OF HPLC TO DEMONSTRATE VARIATION OF VENOM TOXIN COMPOSITION IN THE THAILAND COBRA VENOMS NAJA-NAJA-KAOUTHIA AND NAJA-NAJA-SIAMENSIS

被引：10

作者：

NAMIRANIAN, S ^{[1
]}

HIDER, RC ^{[1
]}

机构：

[1] UNIV LONDON,KINGS COLL,DEPT PHARM,MANRESA RD,LONDON SW3 6LX,ENGLAND

来源：

TOXICON | 1992年 / 30卷 / 01期

关键词：

D O I：

10.1016/0041-0101(92)90501-U

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The composition of the venoms of Naja naja kaouthia and Naja naja siamensis from different commercial sources has been investigated using both ion-exchange and reverse-phase high-pressure liquid chromatography (RP-HPLC) in order to investigate variation in toxin contents. The venoms contained identical major toxin components, although in different relative concentrations. The venom collected separately from the left and right glands of individual snakes were virtually the same as judged by RP-HPLC. The cytotoxin CT-II, which was previously only reported to be present in Naja naja siamensis venom, was detected in all the venoms investigated. Two long neurotoxin homologues have also been isolated.

引用

页码：47 / 61

页数：15

共 28 条

[1]

BOIE H, 1827, ISIS JAVA, V20, P557

[2] CHARACTERIZATION OF ELAPIDAE SNAKE-VENOM COMPONENTS USING OPTIMIZED REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC CONDITIONS AND SCREENING ASSAYS FOR ALPHA-NEUROTOXIN AND PHOSPHOLIPASE-A2 ACTIVITIES [J].

BOUGIS, PE ;

MARCHOT, P ;

ROCHAT, H .

BIOCHEMISTRY, 1986, 25 (22) :7235-7243

[3]

BOULENGER GA, 1896, CATALOGUE SNAKES BRI, V3, P383

[4] ACETYLCHOLINE RECEPTORS AT NEUROMUSCULAR SYNAPSES - PHYLOGENETIC DIFFERENCES DETECTED BY SNAKE ALPHA-NEUROTOXINS [J].

BURDEN, SJ ;

HARTZELL, HC ;

YOSHIKAMI, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (08) :3245-3249

[5] USE OF SULFOPROPYL-SEPHADEX CHROMATOGRAPHY AND DISK GEL-ELECTROPHORESIS FOR FRACTIONATION AND IDENTIFICATION OF ELAPID VENOMS [J].

CHATMAN, VB ;

DIMARI, SJ .

TOXICON, 1974, 12 (04) :405-414

[6]

CHIOU SH, 1989, INT J PEPT PROT RES, V34, P148

[7]

DERANIYAGALA P. E. P., 1960, SPOLIA ZEYLANICA, V29, P41

[8] CONFORMATIONAL PROPERTIES OF THE NEUROTOXINS AND CYTOTOXINS ISOLATED FROM ELAPID SNAKE-VENOMS [J].

DUFTON, MJ ;

HIDER, RC .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1983, 14 (02) :113-171

[9]

FRYKLUND L, 1975, BIOCHEMISTRY-US, V14, P2860, DOI 10.1021/bi00684a011

[10]

GOLAY P, 1985, CHECKLIST KEY TERRES

← 1 2 3 →