HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ALIPHATIC THIOLS WITH AROYLACRYLIC ACIDS AS FLUOROGENIC PRECOLUMN DERIVATIZATION REAGENTS

被引：30
作者：
GATTI, R
CAVRINI, V
ROVERI, P
PINZAUTI, S
机构：
[1] DEPT PHARMACEUT SCI,VIA BELMELORO 6,I-40126 BOLOGNA,ITALY
[2] DEPT PHARMACEUT SCI,I-50121 FLORENCE,ITALY
来源：
JOURNAL OF CHROMATOGRAPHY | 1990年 / 507卷
关键词：
D O I：
10.1016/S0021-9673(01)84224-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The use of the methyl ester of 4-(6-methoxynaphthalen-2-yl)-4-oxo-2-butenoic acid as a fluorogenic labelling regent for the high-performance liquid chromatography (HPLC) of biologically important thiols (glutathione, cysteine, acetylcysteine, homocysteine, cysteamine, sodium 2-mercaptoethanesulphonate and thiola) was investigated. The compound reacts selectively and rapidly (10 min at ambient temperature and pH 7.5) with the thiols to give fluorescent adducts that can be separated by reversed-phase HPLC and detected fluororimetrically (λem = 450 nm; λem = 310 nm). Applications to the determination of l-cysteine and mesna in pharmaceutical formulations are described. © 1990.
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页码：451 / 458
页数：8
相关论文
共 17 条
[1]   REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF THIOL BIMANE DERIVATIVES [J].
BAEYENS, WRG ;
VANDERWEKEN, G ;
DEMOERLOOSE, P .
ANALYTICA CHIMICA ACTA, 1988, 205 (1-2) :43-51
[2]  
CAVRINI V, 1982, FARMACO-ED SCI, V37, P171
[3]   ANALYSIS OF THIOLS BY HPLC AFTER FLUORESCENT PRELABELING WITH 4-(6-METHYLNAPHTHALEN-2-YL)-4-OXOBUTEN-2-OIC ACID [J].
CAVRINI, V ;
GATTI, R ;
ROVERI, P ;
DIPIETRA, A .
CHROMATOGRAPHIA, 1989, 27 (5-6) :185-190
[4]   USE OF 4-(6-METHYLNAPHTHALEN-2-YL)-4-OXOBUT-2-ENOIC ACID AS A REAGENT FOR THE SPECTROPHOTOMETRIC AND FLUORIMETRIC DETERMINATION OF ALIPHATIC THIOL DRUGS [J].
CAVRINI, V ;
GATTI, R ;
ROVERI, P ;
CESARONI, MR .
ANALYST, 1988, 113 (09) :1447-1452
[5]   PRECOLUMN AND POSTCOLUMN DERIVATIZATION CHEMISTRY IN CONJUNCTION WITH HPLC FOR PHARMACEUTICAL ANALYSIS [J].
DANIELSON, ND ;
TARGOVE, MA ;
MILLER, BE .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1988, 26 (08) :362-371
[6]   PHENANTHRYLALKANOIC ACIDS .1. SYNTHESES AND BIOLOGICAL-ACTIVITIES OF 1-PHENANTHRYL DERIVATIVES [J].
EIRIN, A ;
FERNANDEZ, F ;
GOMEZ, G ;
LOPEZ, C ;
SANTOS, A ;
CALLEJA, JM ;
DELAIGLESIA, D ;
CANO, E .
ARCHIV DER PHARMAZIE, 1987, 320 (11) :1110-1118
[7]   SUCCINOYLATION OF 2-METHOXYNAPHTHALENE [J].
GHOSAL, M .
JOURNAL OF ORGANIC CHEMISTRY, 1960, 25 (11) :1856-1859
[8]   DETERMINATION OF N-ACETYLCYSTEINE, INTACT AND OXIDIZED, IN PLASMA BY COLUMN LIQUID-CHROMATOGRAPHY AND POSTCOLUMN DERIVATIZATION [J].
JOHANSSON, M ;
WESTERLUND, D .
JOURNAL OF CHROMATOGRAPHY, 1987, 385 :343-356
[9]   FLUOROMETRIC-DETERMINATION OF 2,3-DIMERCAPTOPROPANE-1-SULFONIC ACID AND OTHER DITHIOLS BY PRECOLUMN DERIVATIZATION WITH BROMOBIMANE AND COLUMN LIQUID-CHROMATOGRAPHY [J].
MAIORINO, RM ;
WEBER, GL ;
APOSHIAN, HV .
JOURNAL OF CHROMATOGRAPHY, 1986, 374 (02) :297-310
[10]  
MARCH J, 1985, ADV ORG CHEM, P670
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