Identification of interacting proteins using the two-hybrid system

被引：0
作者：
Hannon, G
Bartel, P
机构：
[1] COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724
[2] MYRIAD GENET INC,SALT LAKE CITY,UT 84108
来源：
METHODS IN MOLECULAR AND CELLULAR BIOLOGY | 1995年 / 5卷 / 05期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The two-hybrid system is a genetic approach to detect the interaction of proteins by expression in a yeast cell. This technique has found wide use in assaying known proteins for interaction and for searching proteins encoded by gene libraries to identify those that interact with a protein of interest. In particular, discoveries resulting from the application of the two-hybrid system to signal transduction and cell cycle control have led to a more complete understanding of these cellular processes. In this article, we present a didactic outline of the two-hybrid system and its application to searching libraries for interacting proteins. (C) 1996 Wiley-Liss, Inc.
引用
收藏
页码：289 / 297
页数：9
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