IMMUNE-RESPONSES TO STRUCTURAL PROTEINS OF AVIAN INFECTIOUS-BRONCHITIS VIRUS

Chickens were vaccinated with live and inactivated infectious bronchitis virus (IBV), and antibody responses to the individual structural proteins, S1, S2, M and N, followed by ELISA and western blotting. All four structural proteins elicited an antibody response in chicks vaccinated with either live or inactivated IBV. The S1, S2 and N proteins elicited similar titres of antibodies following vaccination with live IBV, whereas the M glycoprotein elicited significantly lower titres. Time of appearance and the course of development of the S1, S2 and N ELISA antibodies were similar, being first detected 2 weeks after vaccination and coincided with appearance of virus neutralizing antibodies. The M antibodies were first detected 4 weeks after vaccination. S1, S2, and N antibody titres were significantly higher in chicks vaccinated at 14 days of age than in chicks vaccinated at either 1 or 7 days of age, and reached maximum levels 4 weeks after the second vaccination. The S1, S2 and N proteins induced cross-reactive antibodies, whereas the M glycoprotein induced antibodies of limited cross-reactivity. Titres of cross-reactive N antibodies were higher than titres of cross-reactive S1 and S2 antibodies, which were similar. Epitopes on the N and S2 proteins that gave rise to cross-reactive antibodies showed the same degree of conservation, whereas the cross-reactive S1 epitopes were marginally less conserved. Vaccination with inactivated virus induced significantly lower antibody titres and at least three vaccinations were necessary for induction of S1, S2, N and M antibodies in all chicks. The S2 glycoprotein was the most immunogenic structural protein following vaccination with inactivated virus. All four proteins induced cell-mediated immune responses in chicks vaccinated with live IBV as determined by a delayed type hypersensitivity response.