TYPING OF HLA-DQA1 AND DQB1 USING DNA SINGLE-STRAND CONFORMATION POLYMORPHISM

被引：65

作者：

CARRINGTON, M ^{[1
]}

MILLER, T ^{[1
]}

WHITE, M ^{[1
]}

GERRARD, B ^{[1
]}

STEWART, C ^{[1
]}

DEAN, M ^{[1
]}

MANN, D ^{[1
]}

机构：

[1] NCI, VIRAL CARCINOGENESIS LAB, FREDERICK, MD 21701 USA

来源：

HUMAN IMMUNOLOGY | 1992年 / 33卷 / 03期

关键词：

D O I：

10.1016/0198-8859(92)90073-V

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The technique of single-strand conformation polymorphism (SSCP), which is capable of distinguishing DNA sequence variability, was adapted to the identification of the HLA-DQA1 and DQB1 alleles. Eight DQA1 alleles and 12 DQB1 alleles were distinguished by amplifying the second exon of the genes in the presence of radioactive deoxynucleotide, denaturing the products with heat, and separating the single strands by electrophoresis in nondenaturing gels. For DQA1, it was possible to distinguish the eight alleles with standard bis-acrylamide or with a Hydrolink gel matrix. Twelve DQB1 alleles were identified by a protocol employing a combination of oligohybridization and SSCP using products amplified by specific DQB1 primers.

引用

页码：208 / 212

页数：5

共 10 条

[1]

BELL JI, 1989, ADV HUM GENET, V18, P1

[2] A MAJOR SEGMENT OF THE NEUROFIBROMATOSIS TYPE-1 GENE - CDNA SEQUENCE, GENOMIC STRUCTURE, AND POINT MUTATIONS [J].

CAWTHON, RM ;

WEISS, R ;

XU, GF ;

VISKOCHIL, D ;

CULVER, M ;

STEVENS, J ;

ROBERTSON, M ;

DUNN, D ;

GESTELAND, R ;

OCONNELL, P ;

WHITE, R .

CELL, 1990, 62 (01) :193-201

[3] MULTIPLE MUTATIONS IN HIGHLY CONSERVED RESIDUES ARE FOUND IN MILDLY AFFECTED CYSTIC-FIBROSIS PATIENTS [J].

DEAN, M ;

WHITE, MB ;

AMOS, J ;

GERRARD, B ;

STEWART, C ;

KHAW, KT ;

LEPPERT, M .

CELL, 1990, 61 (05) :863-870

[4] A SIMPLE AND RAPID METHOD FOR HLA-DP GENOTYPING BY DIGESTION OF PCR-AMPLIFIED DNA WITH ALLELE-SPECIFIC RESTRICTION ENDONUCLEASES [J].

MAEDA, M ;

URYU, N ;

MURAYAMA, N ;

ISHII, H ;

OTA, M ;

TSUJI, K ;

INOKO, H .

HUMAN IMMUNOLOGY, 1990, 27 (02) :111-121

[5] AUTOMATED DNA DIAGNOSTICS USING AN ELISA-BASED OLIGONUCLEOTIDE LIGATION ASSAY [J].

NICKERSON, DA ;

KAISER, R ;

LAPPIN, S ;

STEWART, J ;

HOOD, L ;

LANDEGREN, U .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (22) :8923-8927

[6] DETECTION OF POLYMORPHISMS OF HUMAN DNA BY GEL-ELECTROPHORESIS AS SINGLE-STRAND CONFORMATION POLYMORPHISMS [J].

ORITA, M ;

IWAHANA, H ;

KANAZAWA, H ;

HAYASHI, K ;

SEKIYA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2766-2770

[7] RAPID AND SENSITIVE DETECTION OF POINT MUTATIONS AND DNA POLYMORPHISMS USING THE POLYMERASE CHAIN-REACTION [J].

ORITA, M ;

SUZUKI, Y ;

SEKIYA, T ;

HAYASHI, K .

GENOMICS, 1989, 5 (04) :874-879

[8] ANALYSIS OF ENZYMATICALLY AMPLIFIED BETA-GLOBIN AND HLA-DQ-ALPHA DNA WITH ALLELE-SPECIFIC OLIGONUCLEOTIDE PROBES [J].

SAIKI, RK ;

BUGAWAN, TL ;

HORN, GT ;

MULLIS, KB ;

ERLICH, HA .

NATURE, 1986, 324 (6093) :163-166

[9] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].

SAIKI, RK ;

GELFAND, DH ;

STOFFEL, S ;

SCHARF, SJ ;

HIGUCHI, R ;

HORN, GT ;

MULLIS, KB ;

ERLICH, HA .

SCIENCE, 1988, 239 (4839) :487-491

[10]

Yang S.Y., 1989, IMMUNOBIOLOGY HLA, P11, DOI [10.1007/978-1-4612-3552-1_2, DOI 10.1007/978-1-4612-3552-1_2]

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